Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP81] to Calpain small subunit 1 - BSA and Azide free
Suitable for: IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free
See all Calpain small subunit 1 primary antibodies
Description

Rabbit monoclonal [SP81] to Calpain small subunit 1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, Flow Cytmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human breast carcinoma tissue Flow Cyt: HeLa, NIH/3T3 and PC-12 cells
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Ab238801 is the carrier-free version of ab101683. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab238801 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP81
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Calpain small subunit 1 with ab101683 at 1/100 dilution (0.94 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101683 for 30 mins at room temperature. This image was generated using ab101683, the same clone, but with a different buffer formulation.
Flow Cytometry - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) labeling Calpain small subunit 1 with purified ab101683 at 1/20 dilution (4.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101683).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

Immunohistochemical analysis of Calpain expression in formalin-fixed, paraffin-embedded Human breast carcinoma using ab101683 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab101683).
Flow Cytometry - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

Flow cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) labeling Calpain small subunit 1 with purified ab101683 at 1/20 dilution (4.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101683).
Flow Cytometry - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

Flow cytometry analysis of NIH/3T3 (mouse embryo) labeling Calpain small subunit 1 with purified ab101683 at 1/20 dilution (4.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101683).
Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com