Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP81] to Calpain small subunit 1 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free
    See all Calpain small subunit 1 primary antibodies

  • Description

    Rabbit monoclonal [SP81] to Calpain small subunit 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast carcinoma tissue Flow Cyt: HeLa, NIH/3T3 and PC-12 cells

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab238801 is the carrier-free version of ab101683. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238801 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Calpain small subunit 1 with ab101683 at 1/100 dilution (0.94 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab101683 for 30 mins at room temperature. This image was generated using ab101683, the same clone, but with a different buffer formulation.

  • Flow Cytometry - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)
    Flow Cytometry - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) labeling Calpain small subunit 1 with purified ab101683 at 1/20 dilution (4.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101683).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

    Immunohistochemical analysis of Calpain expression in formalin-fixed, paraffin-embedded Human breast carcinoma using ab101683 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab101683).

  • Flow Cytometry - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)
    Flow Cytometry - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

    Flow cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) labeling Calpain small subunit 1 with purified ab101683 at 1/20 dilution (4.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101683).

  • Flow Cytometry - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)
    Flow Cytometry - Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

    Flow cytometry analysis of NIH/3T3 (mouse embryo) labeling Calpain small subunit 1 with purified ab101683 at 1/20 dilution (4.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101683).

  • Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)
    Anti-Calpain small subunit 1 antibody [SP81] - BSA and Azide free (ab238801)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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