Anti-C9orf40 antibody (ab202286)
Key features and details
- Rabbit polyclonal to C9orf40
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-C9orf40 antibody -
Description
Rabbit polyclonal to C9orf40 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human C9orf40 aa 75-125. The exact sequence is proprietary. NP_060468.2
Database link: Q8IXQ3 -
Positive control
- Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH 3T3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab202286 was affinity purified using an epitope specific to C9orf40 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-C9orf40 antibody (ab202286)All lanes : Anti-C9orf40 antibody (ab202286) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells
Lane 2 : Whole cell lysate from 293T cells
Lane 3 : Whole cell lysate from Jurkat cells
Lane 4 : Whole cell lysate from mouse TCMK-1 cells
Lane 5 : Whole cell lysate from mouse NIH 3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 21 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-C9orf40 antibody (ab202286)Lane 1: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognizes an epitope of C9orf40.
Lane 2: Detection of Human C9orf40 by Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202286 at 6 µg per reaction for IP.
Lane 3: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab202286 at 0.4 µg/ml.Detection: Chemiluminescence with an exposure time
of 3 minutes.