Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-YTHDF1 antibody [EPR22349-16] (ab252346)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22349-16] to YTHDF1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-YTHDF1 antibody [EPR22349-16]
    See all YTHDF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22349-16] to YTHDF1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type mESC, NIH/3T3 and PC-12 whole cell lysates; mouse and rat testis lysate. IHC-P: Mouse and rat cerebrum tissue. Flow Cyt: NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22349-16

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF1 antibody [EPR22349-16] (ab252346)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF1 antibody [EPR22349-16] (ab252346)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling YTHDF1 with ab252346 at 1/100 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum (PMID:29103884; 26046440) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Perform heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF1 antibody [EPR22349-16] (ab252346)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF1 antibody [EPR22349-16] (ab252346)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling YTHDF1 with ab252346 at 1/100 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrum (PMID:29103884; 26046440) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Perform heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

  • Flow Cytometry - Anti-YTHDF1 antibody [EPR22349-16] (ab252346)
    Flow Cytometry - Anti-YTHDF1 antibody [EPR22349-16] (ab252346)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cell line labeling YTHDF1 with ab252346 at 1/40 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-YTHDF1 antibody [EPR22349-16] (ab252346)
    Western blot - Anti-YTHDF1 antibody [EPR22349-16] (ab252346)
    All lanes : Anti-YTHDF1 antibody [EPR22349-16] (ab252346) at 1/1000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Rat testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa


    Exposure time: 48 seconds


    Recommended to use fresh lysate to avoid target protein being degraded.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-YTHDF1 antibody [EPR22349-16] (ab252346)
    Western blot - Anti-YTHDF1 antibody [EPR22349-16] (ab252346)
    All lanes : Anti-YTHDF1 antibody [EPR22349-16] (ab252346) at 1/1000 dilution

    Lane 1 : Wild-type mESC (mouse embryo stem cell) whole cell lysate at 20 µg
    Lane 2 : YTHDF1 knockout mESC whole cell lysate at 20 µg
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa



    The wild-type and YTHDF1 knockout cell lysates were kindly provided by an anonymous collaborator.

    ab252346 was shown to specifically react with YTHDF1 in wild-type mESC cells as signal was lost in YTHDF1 knockout cells. Wild-type and YTHDF1 knockout samples were subjected to SDS-PAGE. ab252346 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Exposure times: Lanes 1-3: 3 minutes; Lane 4: 20 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-YTHDF1 antibody [EPR22349-16] (ab252346)
    Anti-YTHDF1 antibody [EPR22349-16] (ab252346)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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