Anti-C9 antibody (ab67040)
Key features and details
- Rabbit polyclonal to C9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C9 antibody
See all C9 primary antibodies -
Description
Rabbit polyclonal to C9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human C9 aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab94838) -
Positive control
- Recombinant Human C9 protein (ab114199) can be used as a positive control in WB. This antibody gave a positive signal in the following tissue lysates: Human placenta; Human plasma total protein
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 63 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. -
Sequence similarities
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 1 TSP type-1 domain. -
Post-translational
modificationsThrombin cleaves factor C9 to produce C9a and C9b.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore. - Information by UniProt
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Database links
- Entrez Gene: 735 Human
- Omim: 120940 Human
- SwissProt: P02748 Human
- Unigene: 654443 Human
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Alternative names
- ARMD15 antibody
- C9 antibody
- C9 deficiency antibody
see all
Images
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Western blot - Anti-C9 antibody (ab67040)All lanes : Anti-C9 antibody (ab67040) at 1 µg/ml
Lane 1 : Human Plasma Total Protein Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Human Complement component C9 precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 74 kDa band observed is also comparable to the molecular weight seen with other commercially available antibodies to C9.
Datasheets and documents
References (0)
ab67040 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-C9 antibody (ab67040)All lanes : Anti-C9 antibody (ab67040) at 1 µg/ml
Lane 1 : Human Plasma Total Protein Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Human Complement component C9 precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 74 kDa band observed is also comparable to the molecular weight seen with other commercially available antibodies to C9.
