Anti-C5a-R antibody (ab59390)
Key features and details
- Rabbit polyclonal to C5a-R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C5a-R antibody
See all C5a-R primary antibodies -
Description
Rabbit polyclonal to C5a-R -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total C5a-R protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human C5a-R. non-phosphopeptide derived from human C5a-R around the phosphorylation site of serine 338.
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Positive control
- WB: Extracts from HeLa cells treated with PMA (125ng/ml, 30mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-C5a-R antibody (ab59390)All lanes : Anti-C5a-R antibody (ab59390) at 1/500 dilution
Lane 1 : Extracts from HeLa cells, treated
with PMA (125ng/ml, 30mins).
Lane 2 : Extracts from HeLa cells, treated
with PMA (125ng/ml, 30mins) plus peptide.
Predicted band size: 39 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody (ab59390)ab59390 staining C5a-R in human lung.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
