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Immunology Innate Immunity Complement Classical Pathway

Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free (ab250583)

Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free (ab250583)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14781(B)] to C4BPA - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free
    See all C4BPA primary antibodies
  • Description

    Rabbit monoclonal [EPR14781(B)] to C4BPA - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250583 is the carrier-free version of ab182140. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250583 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14781(B)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway

Images

  • Western blot - Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free (ab250583)
    Western blot - Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free (ab250583)
    Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140) at 1/10000 dilution + Human plasma lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab182140, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free (ab250583)
    Western blot - Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free (ab250583)
    Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140) at 1/2000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 67 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    This data was developed using ab182140, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free (ab250583)
    Immunoprecipitation - Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free (ab250583)
    This data was developed using ab182140, the same antibody clone in a different buffer formulation.Western blot analysis of C4BPA in Human plasma immunoprecipitated with ab182140 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.
  • Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free (ab250583)
    Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free (ab250583)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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