Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C4b antibody [EPR11203-27] - BSA and Azide free (ab250455)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11203-27] to C4b - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-C4b antibody [EPR11203-27] - BSA and Azide free
    See all C4b primary antibodies

  • Description

    Rabbit monoclonal [EPR11203-27] to C4b - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: HepG2 cells; IHC: Human liver tissue; WB: HepG2 whole cell lysate. Human plasma and serum lysates. Human liver, heart and testis tissue lysates.

  • General notes

    Ab250455 is the carrier-free version of ab181241. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250455 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11203-27

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C4b antibody [EPR11203-27] - BSA and Azide free (ab250455)
    Western blot - Anti-C4b antibody [EPR11203-27] - BSA and Azide free (ab250455)
    All lanes : Anti-C4b antibody [EPR11203-27] (ab181241) at 1/10000 dilution (purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
    Lane 2 : Human plasma lysate at 15 µg
    Lane 3 : Human serum lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 193 kDa
    Observed band size: 200,70,93 kDa
    why is the actual band size different from the predicted?



    The molecular weights observed are consistent with what have been described in the literature (PMID: 15632414, 31178241 and 30210498).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181241).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] - BSA and Azide free (ab250455)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] - BSA and Azide free (ab250455)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling C4b with purified ab181241 at 1/75 dilution (9.53 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181241).
  • Western blot - Anti-C4b antibody [EPR11203-27] - BSA and Azide free (ab250455)
    Western blot - Anti-C4b antibody [EPR11203-27] - BSA and Azide free (ab250455)
    All lanes : Anti-C4b antibody [EPR11203-27] (ab181241) at 1/10000 dilution (purified)

    Lane 1 : Human liver lysate
    Lane 2 : Human heart lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 193 kDa
    Observed band size: 200,70,93 kDa why is the actual band size different from the predicted?



    The molecular weights observed are consistent with what have been described in the literature (PMID: 15632414, 31178241 and 30210498).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181241).

  • Immunocytochemistry - Anti-C4b antibody [EPR11203-27] - BSA and Azide free (ab250455)
    Immunocytochemistry - Anti-C4b antibody [EPR11203-27] - BSA and Azide free (ab250455)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling c4b with purified ab181241 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181241).

  • Anti-C4b antibody [EPR11203-27] - BSA and Azide free (ab250455)
    Anti-C4b antibody [EPR11203-27] - BSA and Azide free (ab250455)

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