Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C10 antibody [EPR23475-105] (ab275025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23475-105] to C10
  • Suitable for: IP, IHC-P, IHC-Fr, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-C10 antibody [EPR23475-105]
    See all C10 primary antibodies

  • Description

    Rabbit monoclonal [EPR23475-105] to C10

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse spleen and lung tissue. IHC-Fr: Mouse small intestine and colon tissue. Flow cyt: P388D1 cells (treated with 1ug/ml lipopolysaccharide (LPS) for 4h then add 300ng/ml Brefeldin A for 16h). IP: Mouse small intestine tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23475-105

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] (ab275025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] (ab275025)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling C10 with ab275025 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse spleen (PMID: 15770051). The section was incubated with ab275025 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] (ab275025)
    Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] (ab275025)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse small intestine tissue labeling C10 with ab275025 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse small intestine (PMID: 7579352). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Flow Cytometry - Anti-C10 antibody [EPR23475-105] (ab275025)
    Flow Cytometry - Anti-C10 antibody [EPR23475-105] (ab275025)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized P388D1 (Mouse lymphoma monocyte; macrophage) (treated with 1ug/ml lipopolysaccharide (LPS) for 4h then add 300ng/ml Brefeldin A for 16h) (Red) / Untreated control (Green) cells labelling C10 with ab275025 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-C10 antibody [EPR23475-105] (ab275025)
    Western blot - Anti-C10 antibody [EPR23475-105] (ab275025)
    All lanes : Anti-C10 antibody [EPR23475-105] (ab275025) at 1/1000 dilution

    Lane 1 : His-tagged mouse C10 recombinant protein, 20ng
    Lane 2 : His-tagged mouse MIP-1 gamma (CCL9) recombinant protein (aa 22-122), 50ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    This product does not recognize CCL9.

  • Immunoprecipitation - Anti-C10 antibody [EPR23475-105] (ab275025)
    Immunoprecipitation - Anti-C10 antibody [EPR23475-105] (ab275025)

    C10 was immunoprecipitated from 0.35 mg Mouse small intestine tissue lysate with ab275025 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275025 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse small intestine tissue lysate 10 ug

    Lane 2: ab275025 IP in Mouse small intestine tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275025 in mouse small intestine tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The expression profile observed is consistent with what has been described in the literature (PMID: 19812544).

  • Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] (ab275025)
    Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] (ab275025)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon tissue labeling C10 with ab275025 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse colon (PMID: 7579352). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] (ab275025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] (ab275025)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling C10 with ab275025 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse lung (PMID: 14734772). The section was incubated with ab275025 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-C10 antibody [EPR23475-105] (ab275025)
    Anti-C10 antibody [EPR23475-105] (ab275025)

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