Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

Order now and get it on Wednesday March 10, 2021

Anti-C10 antibody [EPR23475-105] (ab275025)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23475-105] to C10
Suitable for: IP, IHC-P, IHC-Fr, Flow Cyt
Reacts with: Mouse, Rat

Product name

Anti-C10 antibody [EPR23475-105]
See all C10 primary antibodies
Description

Rabbit monoclonal [EPR23475-105] to C10
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IHC-Fr	Mouse
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Mouse spleen and lung tissue. IHC-Fr: Mouse small intestine and colon tissue. Flow cyt: P388D1 cells (treated with 1ug/ml lipopolysaccharide (LPS) for 4h then add 300ng/ml Brefeldin A for 16h). IP: Mouse small intestine tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23475-105
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] (ab275025)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling C10 with ab275025 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse spleen (PMID: 15770051). The section was incubated with ab275025 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] (ab275025)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse small intestine tissue labeling C10 with ab275025 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse small intestine (PMID: 7579352). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Flow Cytometry - Anti-C10 antibody [EPR23475-105] (ab275025)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized P388D1 (Mouse lymphoma monocyte; macrophage) (treated with 1ug/ml lipopolysaccharide (LPS) for 4h then add 300ng/ml Brefeldin A for 16h) (Red) / Untreated control (Green) cells labelling C10 with ab275025 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-C10 antibody [EPR23475-105] (ab275025)

All lanes : Anti-C10 antibody [EPR23475-105] (ab275025) at 1/1000 dilution

Lane 1 : His-tagged mouse C10 recombinant protein, 20ng
Lane 2 : His-tagged mouse MIP-1 gamma (CCL9) recombinant protein (aa 22-122), 50ng

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 13 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 8 seconds.

This product does not recognize CCL9.
Immunoprecipitation - Anti-C10 antibody [EPR23475-105] (ab275025)

C10 was immunoprecipitated from 0.35 mg Mouse small intestine tissue lysate with ab275025 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275025 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

Lane 1: Mouse small intestine tissue lysate 10 ug

Lane 2: ab275025 IP in Mouse small intestine tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275025 in mouse small intestine tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.

The expression profile observed is consistent with what has been described in the literature (PMID: 19812544).
Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] (ab275025)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon tissue labeling C10 with ab275025 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse colon (PMID: 7579352). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 (2 ug/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] (ab275025)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling C10 with ab275025 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse lung (PMID: 14734772). The section was incubated with ab275025 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-C10 antibody [EPR23475-105] (ab275025)

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