Human I-309 ELISA Kit (CCL1) (ab100536)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.12 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human I-309 ELISA Kit (CCL1) -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
4.12 pg/ml - 3000 pg/mlRecovery
95 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 79.9 70% - 103% Plasma 97.65 88% - 110% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s I-309 (CCL-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human I-309 (CCL-1) in plasma and cell culture supernatants. (Human I-309 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human I-309 coated on a 96-well plate. Standards and samples are pipetted into the wells and I-309 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human I-309 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-309 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human I-309 in normal serum with ab100536, only in serum spiked with Human I-309.
Notes
Optimization may be required with urine samples
Platform
MicroplateProperties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-Human I-309 (lyophilized) 2 vials I-309 Microplate (12 x 8 wells) 1 unit Recombinant Human I-309 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- C-C motif chemokine 1
- Ccl1
- CCL1_HUMAN
see all -
Database links
- Entrez Gene: 6346 Human
- Omim: 182281 Human
- SwissProt: P22362 Human
- Unigene: 72918 Human
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-Human I-309 (lyophilized) 2 vials I-309 Microplate (12 x 8 wells) 1 unit Recombinant Human I-309 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- C-C motif chemokine 1
- Ccl1
- CCL1_HUMAN
see all -
Database links
- Entrez Gene: 6346 Human
- Omim: 182281 Human
- SwissProt: P22362 Human
- Unigene: 72918 Human
Images
-
Typical standard curve
Representative standard curve using ab100536