Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR045F] to c-Maf - BSA free
Suitable for: ICC, IHC-P, IP, WB
Reacts with: Mouse, Human

Product name

Anti-c-Maf antibody [BLR045F] - BSA free
See all c-Maf primary antibodies
Description

Rabbit monoclonal [BLR045F] to c-Maf - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Mouse Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human c-Maf aa 125-175. The exact sequence is proprietary. NP_005351.2 and Gene ID 4094.
Database link: O75444
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR045F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

c-Maf was immunoprecipitated from 1.0 mg RPMI 8226 whole cell lysate with ab243901 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243901 at 1/1000 dilution.
Lane 1: ab243901 IP in RPMI 8226 whole cell lysate.

Lane 2: Control IgG in RPMI 8226 whole cell lysate.

Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Detection of human and mouse cMaf by WB of HEK293T transfected with myc tagged human or mouse MafA, MafB or cMaf using ab243901. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower panel: Rabbit anti-Myc.
Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Western blot analysis using ab243901 at 1/1000 dilution.
Lane 1: HeLa cell whole cell lysate (50 µg).
Lane 2: HEK-293T cell whole cell lysate (50 µg).
Lane 3: A549 whole cell lysate (50 µg).
Lane 4: RPMI 8226 whole cell lysate (50 µg).
Lane 5: HepG2 whole cell lysate (50 µg).
Lane 6: Jurkat whole cell lysate (50 µg).
Lane 7: 786-O whole cell lysate (50 µg).
Lane 8: MCF7 whole cell lysate (50 µg).
Lane 9: K562 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Formalin-fixed, paraffin-embedded RPMI 8226 cells c-Mafa using ab243901 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for c-Maf using ab243901 at 1/125 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for c-Maf using ab243901 at 1/125 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com