Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9980(2)] to BTF - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BTF antibody [EPR9980(2)] - BSA and Azide free
    See all BTF primary antibodies

  • Description

    Rabbit monoclonal [EPR9980(2)] to BTF - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon tissue.

  • General notes

    Ab236159 is the carrier-free version of ab181240. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236159 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
    Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling BTF with Purified ab181240 at 1:1000 dilution (1.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody.

    Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody.

    Hematoxylin was used as a counterstain

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric cancer tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody.

    Hematoxylinwas used as a counterstain

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

  • Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
    Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling BTF with unpurified ab181240 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)

    Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling BTF with unpurified ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BTF with ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

  • Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
    Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)

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