Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BTF antibody [EPR9980(2)] (ab181240)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9980(2)] to BTF
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BTF antibody [EPR9980(2)]
    See all BTF primary antibodies

  • Description

    Rabbit monoclonal [EPR9980(2)] to BTF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, RAW64.7, PC-12, Jurkat and MCF-7 whole cell lysates ICC/IF: HepG2 and MCF-7 cells IHC-P: Human colon, gastric cancer and ovarian cancer, mouse testis, and rat liver tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Western blot - Anti-BTF antibody [EPR9980(2)] (ab181240)
    All lanes : Anti-BTF antibody [EPR9980(2)] (ab181240) at 1.5 µg/ml (purified)

    Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 2 : RAW64.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 106 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling BTF with Purified ab181240 at 1:1000 dilution (1.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric cancer tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
  • Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] (ab181240)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling BTF with unpurified ab181240 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Western blot - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Western blot - Anti-BTF antibody [EPR9980(2)] (ab181240)
    All lanes : Anti-BTF antibody [EPR9980(2)] (ab181240) at 1/10000 dilution (Unpurified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : MCF-7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat anti-Rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
    Lanes 2-3 : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 106 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BTF with unpurified ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)

    Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling BTF with unpurified ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

  • Anti-BTF antibody [EPR9980(2)] (ab181240)
    Anti-BTF antibody [EPR9980(2)] (ab181240)

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