Anti-BRN4 antibody (ab104562)
Key features and details
- Rabbit polyclonal to BRN4
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-BRN4 antibody
See all BRN4 primary antibodies -
Description
Rabbit polyclonal to BRN4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Rabbit, Horse, Cow, Human, Pig, Macaque monkey, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Rat BRN4 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab124139) -
Positive control
- This antibody gave a positive signal in the following tissue lysayes: Rat Brain; Rat Hippocampus; Rat Cerebellum.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab104562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). IHC-P Use a concentration of 5 µg/ml. Target
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Function
Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain. -
Tissue specificity
Brain specific. -
Involvement in disease
Defects in POU3F4 are a cause of deafness X-linked type 2 (DFNX2) [MIM:304400]. A mixed type of deafness characterized by both conductive hearing loss resulting from stapes (perilymphatic gusher) fixation and progressive sensorineural deafness. -
Sequence similarities
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5456 Human
- Entrez Gene: 18994 Mouse
- Entrez Gene: 29589 Rat
- Omim: 300039 Human
- SwissProt: P49335 Human
- SwissProt: P62515 Mouse
- SwissProt: P62516 Rat
- Unigene: 2229 Human
see all -
Alternative names
- Brain specific homeobox POU domain protein 4 antibody
- Brain-4 antibody
- Brain-specific homeobox/POU domain protein 4 antibody
see all
Images
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Western blot - Anti-BRN4 antibody (ab104562)All lanes : Anti-BRN4 antibody (ab104562) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate
Lane 2 : Cerebellum Rat Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 190 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRN4 antibody (ab104562)IHC image of BRN4 staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104562, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (0)
ab104562 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-BRN4 antibody (ab104562)All lanes : Anti-BRN4 antibody (ab104562) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate
Lane 2 : Cerebellum Rat Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 190 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRN4 antibody (ab104562)
IHC image of BRN4 staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104562, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
