Anti-BrdU antibody [Bu20a] (ab136650)
![Anti-BrdU antibody [Bu20a] (ab136650)](https://www.abcam.com/ps/products/136/ab136650/Images/ab136650-206375-anti-brdu-antibody-bu20a-immunofluorescence.jpg "Anti-BrdU antibody [Bu20a] (ab136650)")
Key features and details
- Mouse monoclonal [Bu20a] to BrdU
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-BrdU antibody [Bu20a]
See all BrdU primary antibodies -
Description
Mouse monoclonal [Bu20a] to BrdU -
Host species
Mouse -
Specificity
ab136650 reacts specifically with BrdU incorporated into DNA during S phase of a cell cycle. -
Tested applications
Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
BrdU conjugated to BSA.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab136650 was purified from cell culture supernatant and is > 95% pure (by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
Bu20a -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [Bu20a] (ab136650)
ICC/IF image of ab136650 stained HeLa cells, both BrdU treated (left image) and normal cells (right image). The cells were 100% methanol fixed (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab136650, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Positive staining can be seen in the BrdU treated cells, but not in the normal cells, demonstrating specificity for BrdU.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [Bu20a] (ab136650)](https://www.abcam.com/ps/products/136/ab136650/Images/ab136650-170779-anti-brdu-antibody-bu20a-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [Bu20a] (ab136650)IHC image of ab136650 staining, both in normal and BrdU treated rat liver formalin fixed paraffin embedded tissue sections, performed on a Leica Bond
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
