Anti-Bordetella pertussis toxin/ptxD antibody (ab188414)
Key features and details
- Rabbit polyclonal to Bordetella pertussis toxin/ptxD
- Suitable for: Dot blot, IP, Neutralising, ELISA, WB
- Reacts with: Bordetella pertussis
- Isotype: IgG
Overview
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Product name
Anti-Bordetella pertussis toxin/ptxD antibody
See all Bordetella pertussis toxin/ptxD primary antibodies -
Description
Rabbit polyclonal to Bordetella pertussis toxin/ptxD -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IP, Neutralising, ELISA, WBmore details -
Species reactivity
Reacts with: Bordetella pertussis -
Immunogen
Full length native protein (purified) corresponding to Bordetella pertussis toxin/ptxD. (Immunization was initiated with toxoid and boosted with native toxin).
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Positive control
- Culture medium of Bordetella pertussis; Purified Bordetella pertussis toxin/ptxD.
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General notes
This product was previously labelled as Bordetella pertussis toxin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Bordetella pertussis toxin/ptxD antibody (ab188414)All lanes : Anti-Bordetella pertussis toxin/ptxD antibody (ab188414) at 1/2000 dilution
Lane 1 : Culture medium of Bordetella pertussis
Lane 2 : Purified Bordetella pertussis toxin/ptxD at 0.01 µgThe toxin consists of five subunits as indicated by S1 to S5.
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ELISA - Anti-Bordetella pertussis toxin/ptxD antibody (ab188414)ELISA analysis of Bordetella pertussis toxin/ptxD, using ab188414. Plate was coated with 100 μg of pertussis toxin per well and 100 μl of ab188414 at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as a secondary antibody. Color was developed with TMB as substrate.
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ELISA - Anti-Bordetella pertussis toxin/ptxD antibody (ab188414)ELISA analysis of Bordetella pertussis toxin/ptxD, using ab188414 at 1/12500 dilution. Plate was coated with indicated amounts of pertussis toxin per well and 100 μl of ab188414 was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as a secondary antibody. Color was developed with TMB as substrate. Dynamic range was 100 pg to 10 ng under these conditions.