Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BOB1 antibody [EP3300] - BSA and Azide free (ab247428)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3300] to BOB1 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BOB1 antibody [EP3300] - BSA and Azide free
    See all BOB1 primary antibodies

  • Description

    Rabbit monoclonal [EP3300] to BOB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247428 is the carrier-free version of ab76010 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247428 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-BOB1 antibody [EP3300] - BSA and Azide free (ab247428)
    Flow Cytometry - Anti-BOB1 antibody [EP3300] - BSA and Azide free (ab247428)
    This data was developed using ab76010, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling BOB1 with unpurified ab76010 at 1/40 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Western blot - Anti-BOB1 antibody [EP3300] - BSA and Azide free (ab247428)
    Western blot - Anti-BOB1 antibody [EP3300] - BSA and Azide free (ab247428)
    Anti-BOB1 antibody [EP3300] (ab76010) at 1/30000 dilution + Ramos cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76010, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EP3300] - BSA and Azide free (ab247428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EP3300] - BSA and Azide free (ab247428)
    This data was developed using ab76010, the same antibody clone in a different buffer formulation.ab76010, at 1/100 dilution, staining BOB1 in human tonsil by immunohistochemistry using paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-BOB1 antibody [EP3300] - BSA and Azide free (ab247428)
    OI-RD Scanning - Anti-BOB1 antibody [EP3300] - BSA and Azide free (ab247428)
    This data was developed using ab76010, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-BOB1 antibody [EP3300] - BSA and Azide free (ab247428)
    Anti-BOB1 antibody [EP3300] - BSA and Azide free (ab247428)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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