Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP742(2)Y] to beta Catenin (phospho S37) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free
    See all beta Catenin primary antibodies

  • Description

    Rabbit monoclonal [EP742(2)Y] to beta Catenin (phospho S37) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects beta Catenin phosphorylated on serine 37.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247399 is the carrier-free version of ab75777 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247399 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)
    Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)
    All lanes : Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/5000 dilution

    Lane 1 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate
    Lane 2 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate treated with MG-132.
    Lane 3 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate treated with MG-132 then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab75777, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)
    Dot Blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)
    This data was developed using ab75777, the same antibody clone in a different buffer formulation.Dot blot analysis of Beta catenin phospho peptide with ab75777 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST. Lane 1: Beta catenin (pS33+pS37) phospho peptide
    Lane 2: Beta catenin (pS33) phospho peptide
    Lane 3: Beta catenin (pS37) phospho peptide
    Lane 4: Beta catenin non-phospho peptide
  • Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)
    Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)
    All lanes : Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/500 dilution

    Lane 1 : 293T cell lysates, untreated
    Lane 2 : 293T cell lysates, treated with calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 86 kDa
    Observed band size: 86 kDa



    This data was developed using ab75777, the same antibody clone in a different buffer formulation.

     

  • Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)
    Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)

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