Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BCMA antibody [EPRBOB-R1-F1-24] - BSA and Azide free (ab254206)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPRBOB-R1-F1-24] to BCMA - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BCMA antibody [EPRBOB-R1-F1-24] - BSA and Azide free
    See all BCMA primary antibodies

  • Description

    Rabbit monoclonal [EPRBOB-R1-F1-24] to BCMA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IP, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: U266B1 cells. IP: U266B1 whole cell lysate. Flow Cyt: U266B1 cells.

  • General notes

    Ab254206 is the carrier-free version of ab245940. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254206 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPRBOB-R1-F1-24] - BSA and Azide free (ab254206)
    Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPRBOB-R1-F1-24] - BSA and Azide free (ab254206)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U266B1 (human multiple myeloma B lymphocyte) cells labeling BCMA with ab245940 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in U266B1 cell line. Negative control cells: Jurkat (PMID: 11104810. The nuclear counterstain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245940).
  • Immunoprecipitation - Anti-BCMA antibody [EPRBOB-R1-F1-24] - BSA and Azide free (ab254206)
    Immunoprecipitation - Anti-BCMA antibody [EPRBOB-R1-F1-24] - BSA and Azide free (ab254206)

    BCMA was immunoprecipitated from 0.35 mg U266B1 (human multiple myeloma B lymphocyte) whole cell lysate with ab245940 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245940 at 1/300 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: U266B1 whole cell lysate 10 µg (Input).
    Lane 2:ab245940 IP in U266B1 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245940 in U266B1 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245940).

  • Flow Cytometry - Anti-BCMA antibody [EPRBOB-R1-F1-24] - BSA and Azide free (ab254206)
    Flow Cytometry - Anti-BCMA antibody [EPRBOB-R1-F1-24] - BSA and Azide free (ab254206)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized U266B1 (human multiple myeloma B lymphocyte) cell line labeling BCMA with ab245940 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245940).
  • Anti-BCMA antibody [EPRBOB-R1-F1-24] - BSA and Azide free (ab254206)
    Anti-BCMA antibody [EPRBOB-R1-F1-24] - BSA and Azide free (ab254206)

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