Anti-Bcl-XL antibody (ab2568)
Key features and details
- Rabbit polyclonal to Bcl-XL
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bcl-XL antibody
See all Bcl-XL primary antibodies -
Description
Rabbit polyclonal to Bcl-XL -
Host species
Rabbit -
Specificity
This antibody detects the 30 kDa Bcl-XL protein. It does not react with other Bcl-2 family members. -
Tested Applications & Species
See all applications and species dataApplication Species ICC Human
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Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-Bcl-XL antibody (ab2568)ICC/IF image of ab2568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry - Anti-Bcl-XL antibody (ab2568) This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappaab2568 staining BCL-XL (green) in Mouse RAW 264.7 cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized by 0.1% Triton X-100 in 2% BSA for 15 minutes and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in 2% BSA in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody.
WGA (red) = Alexa Fluor®568-conjugated Wheat Germ Agglutinin
