Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [NOR 235J] to Bcl-2 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free
    See all Bcl-2 primary antibodies

  • Description

    Rat monoclonal [NOR 235J] to Bcl-2 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human tonsil tissue.

  • General notes

    ab252802 is the PBS only version of ab241548.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802)

    This image was produced using the same clone but in a different formulation, ab241548.

    IHC image of Bcl-2 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BOND™ system using the standard Protocol F.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab241548, 0.98 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

     

  • Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802)
    Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802)

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