Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BCKDK antibody [EPR7564] (ab128935)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7564] to BCKDK
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BCKDK antibody [EPR7564]
    See all BCKDK primary antibodies

  • Description

    Rabbit monoclonal [EPR7564] to BCKDK

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human BCKDK aa 50-150. The exact sequence is proprietary.
    Database link: O14874

  • Positive control

    • WB: HeLa, Rat heart, Rat kidney, PC-12, and RAW 264.7 lysates.

  • General notes

    This product was previously labelled as BCKDH kinase

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-BCKDK antibody [EPR7564] (ab128935)
    Western blot - Anti-BCKDK antibody [EPR7564] (ab128935)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: BCKDH kinase knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab128935 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab128935 was shown to specifically react with BCKDH kinase when BCKDH kinase knockout samples were used. Wild-type and BCKDH kinase knockout samples were subjected to SDS-PAGE.  Ab128935 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-BCKDK antibody [EPR7564] (ab128935)
    Western blot - Anti-BCKDK antibody [EPR7564] (ab128935)
    All lanes : Anti-BCKDK antibody [EPR7564] (ab128935) at 1/1000 dilution (Purified)

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Western blot - Anti-BCKDK antibody [EPR7564] (ab128935)
    Western blot - Anti-BCKDK antibody [EPR7564] (ab128935)
    All lanes : Anti-BCKDK antibody [EPR7564] (ab128935) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Rat heart lysates
    Lane 3 : Rat kidney lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Western blot - Anti-BCKDK antibody [EPR7564] (ab128935)
    Western blot - Anti-BCKDK antibody [EPR7564] (ab128935)
    All lanes : Anti-BCKDK antibody [EPR7564] (ab128935) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal kidney lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • OI-RD Scanning - Anti-BCKDK antibody [EPR7564] (ab128935)
    OI-RD Scanning - Anti-BCKDK antibody [EPR7564] (ab128935)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-BCKDK antibody [EPR7564] (ab128935)
    Anti-BCKDK antibody [EPR7564] (ab128935)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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