Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Baf180 antibody [BL-39-2C3] (ab243876)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-39-2C3] to Baf180
Suitable for: WB, IHC-P, ICC, IP
Reacts with: Mouse, Human

Product name

Anti-Baf180 antibody [BL-39-2C3]
See all Baf180 primary antibodies
Description

Rabbit monoclonal [BL-39-2C3] to Baf180
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Baf180 aa 1-293.
Database link: Q86U86
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-39-2C3
Isotype

IgG
Research areas

Immunocytochemistry - Anti-Baf180 antibody [BL-39-2C3] (ab243876)

Formaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Baf180 using ab243876 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Baf180 antibody [BL-39-2C3] (ab243876)

Formalin-fixed, paraffin-embedded human Breast tissue stained for Baf180 using ab243876 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunoprecipitation - Anti-Baf180 antibody [BL-39-2C3] (ab243876)

Baf180 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243876 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243876 at 1/1000 dilution.
Lane 1: ab243876 IP in HEK-293T whole cell lysate.

Lane 2: Contol IgG in HEK-293T whole cell lysate.

Detection: Chemiluminescence with an exposure time of 3 minutes.
Western blot - Anti-Baf180 antibody [BL-39-2C3] (ab243876)

Western blot analysis using ab243876 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (15 µg).
Lane 2: HEK-293T whole cell lysate (15 µg).
Lane 3: MCF7 whole cell lysate (15 µg).
Lane 4: HepG2 whole cell lysate (15 µg).
Lane 5: A549 whole cell lysate (15 µg).
Lane 6: SW620 whole cell lysate (15 µg).
Lane 7: SK whole cell lysate (15µg).
Lane 8: Jurkat whole cell lysate (15 µg).
Lane 9: TCMK-1 whole cell lysate (15 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds
Anti-Baf180 antibody [BL-39-2C3] (ab243876)

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