Anti-BACH1.3 antibody [OTI4E11] (ab128486)
![Anti-BACH1.3 antibody [OTI4E11] (ab128486)](https://www.abcam.com/ps/products/128/ab128486/Images/ab128486-142246-anti-bach13-antibody-oti4e11-western-blot.jpg "Anti-BACH1.3 antibody [OTI4E11] (ab128486)")
Key features and details
- Mouse monoclonal [OTI4E11] to BACH1.3
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-BACH1.3 antibody [OTI4E11]
See all BACH1.3 primary antibodies -
Description
Mouse monoclonal [OTI4E11] to BACH1.3 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human BACH1.3 aa 1-736. produced in HEK293T cells (NP_996749).
Sequence:MSLSENSVFAYESSVHSTNVLLSLNDQRKKDVLCDVTIFVEGQRFRAHRS VLAACSSYFHSRIVGQADGELNITLPEEVTVKGFEPLIQFAYTAKLILSK ENVDEVCKCVEFLSVHNIEESCFQFLKFKFLDSTADQQECPRKKCFSSHC QKTDLKLSLLDQRDLETDEVEEFLENKNVQTPQCKLRRYQGNAKASPPLQ DSASQTYESMCLEKDAALALPSLCPKYRKFQKAFGTDRVRTGESSVKDIH ASVQPNERSENECLGGVPECRDLQVMLKCDESKLAMEPEETKKDPASQCP TEKSEVTPFPHNSSIDPHGLYSLSLLHTYDQYGDLNFAGMQNTTVLTEKP LSGTDVQEKTFGESQDLPLKSDLGTREDSSVASSDRSSVEREVAEHLAKG FWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQ RTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISL GDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKL TPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLL KERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSF LISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQ MSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE
Database link: O14867 -
Positive control
- WB: HEK-293T cells transfected with BACH1.3. IHC-P: Human bladder carcinoma and tonsil tissues. Flow: BACH1 transfected HEK-293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI4E11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-BACH1.3 antibody [OTI4E11] (ab128486)All lanes : Anti-BACH1.3 antibody [OTI4E11] (ab128486) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY BACH1.3 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 82 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)](https://www.abcam.com/ps/products/128/ab128486/Images/ab128486-142245-anti-bach13-antibody-oti4e11-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)Paraffin-embedded human bladder carcinoma tissue stained for BACH1.3 using ab128486 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)](https://www.abcam.com/ps/products/128/ab128486/Images/ab128486-142244-anti-bach13-antibody-oti4e11-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACH1.3 antibody [OTI4E11] (ab128486)Paraffin-embedded human tonsil tissue stained for BACH1.3 using ab128486 at 1/150 dilution in immunohistochemical analysis.
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![Flow Cytometry - Anti-BACH1.3 antibody [OTI4E11] (ab128486)](https://www.abcam.com/ps/products/128/ab128486/Images/ab128486-142243-anti-bach13-antibody-oti4e11-flow-cytometry.jpg)
Flow Cytometry - Anti-BACH1.3 antibody [OTI4E11] (ab128486)Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY BACH1.3 (Red) or empty vector control plasmid (Blue) stained for BACH1.3 using ab128486 at 1/100 dilution.
