Anti-ATP1B1 antibody [464.8] (ab8344)
![Anti-ATP1B1 antibody [464.8] (ab8344)](https://www.abcam.com/ps/products/8/ab8344/Images/ab8344-169163-anti-beta-1-sodium-potassium-atpase-antibody-4648-western-blot.jpg "Anti-ATP1B1 antibody [464.8] (ab8344)")
Key features and details
- Mouse monoclonal [464.8] to ATP1B1
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-ATP1B1 antibody [464.8]
See all ATP1B1 primary antibodies -
Description
Mouse monoclonal [464.8] to ATP1B1 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Dog, Pig
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Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human kidney tissue lysates Flow Cyt: HepG2 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Contains 0.4M arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
464.8 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-ATP1B1 antibody [464.8] (ab8344)Anti-ATP1B1 antibody [464.8] (ab8344) at 1/500 dilution + Human kidney tissue lysate - total protein (ab30203) at 20 µg
Secondary
Granzyme B overexpression 293T lysate (whole cell) (ab94040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
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![Flow Cytometry - Anti-ATP1B1 antibody [464.8] (ab8344)](https://www.abcam.com/ps/products/8/ab8344/Images/ab8344-14142-anti-beta-1-sodium-potassium-atpase-antibody-4648-flow-cytometry.jpg)
Flow Cytometry - Anti-ATP1B1 antibody [464.8] (ab8344)Overlay histogram showing HepG2 cells stained with ab8344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8344, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
