Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (ab109867)
![Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (ab109867)](https://www.abcam.com/ps/products/109/ab109867/Images/ab109867-122859-anti-atp-synthase-immunocapture-antibody-12f4ad8af8-flow-cytometry.jpg "Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (ab109867)")
Key features and details
- Mouse monoclonal [12F4AD8AF8] to ATP synthase Immunocapture
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] -
Description
Mouse monoclonal [12F4AD8AF8] to ATP synthase Immunocapture -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC or WB -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein. This information is considered to be commercially sensitive.
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Positive control
- Bovine heart mitochondria ab110338
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General notes
ab109867 is a sample of pure immunocapture antibody, not immobilized to a solid support.
This antibody was tested and confirmed not suitable for WB and ICC.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
The purity of ab109867 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum free medium, and then purified by biochemical fractionation. -
Primary antibody notes
ab109867 is a sample of pure immunocapture antibody, not immobilized to a solid support. -
Clonality
Monoclonal -
Clone number
12F4AD8AF8 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (ab109867)Overlay histogram showing HepG2 cells stained with ab109867 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109867, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
