Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (ab109867)

Key features and details

  • Mouse monoclonal [12F4AD8AF8] to ATP synthase Immunocapture
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-ATP synthase Immunocapture antibody [12F4AD8AF8]

  • Description

    Mouse monoclonal [12F4AD8AF8] to ATP synthase Immunocapture

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein. This information is considered to be commercially sensitive.

  • Positive control

  • General notes

    ab109867 is a sample of pure immunocapture antibody, not immobilized to a solid support.

    This antibody was tested and confirmed not suitable for WB and ICC.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Images

  • Flow Cytometry - Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (ab109867)
    Flow Cytometry - Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (ab109867)
    Overlay histogram showing HepG2 cells stained with ab109867 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109867, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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