All lanes : Anti-ATG14L antibody (ab227849) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : RD whole cell extract
Lane 3 : Mouse brain extract
Lane 4 : Rat brain extract

Lysates/proteins at 60 µg per lane.

Predicted band size: 55 kDa

7.5% SDS-PAGE gel.

Paraffin-embedded HeLa xenograft tissue stained for ATG14L using ab227849 at 1/500 dilution in immunohistochemical analysis.

All lanes : Anti-ATG14L antibody (ab227849) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

Lysates/proteins at 30 µg per lane.

Developed using the ECL technique.

Predicted band size: 55 kDa

10% SDS-PAGE gel.