Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)
![Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)](https://www.abcam.com/ps/products/247/ab247876/Images/ab247876-408152-anti-atg12-antibody-epr4800-bsa-and-azide-free-western-blot.jpg "Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4800] to ATG12 - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ATG12 antibody [EPR4800] - BSA and Azide free
See all ATG12 primary antibodies -
Description
Rabbit monoclonal [EPR4800] to ATG12 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247876 is the carrier-free version of ab109491. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247876 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4800 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)
This data was developed using ab109491, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ATG12 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HCT116 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109491 observed at 55 kDa. Observed band shows ATG12-ATG5 conjugation. Red - loading control, ab8245, observed at 37 kDa.
Ab109491 was shown to specifically react with ATG12 in wild-type cells along with additional cross-reactive bands. The band was not seen in ATG12 knockout HAP1 cells. Wild-type and ATG12 knockout samples were subjected to SDS-PAGE. ab109491 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)](https://www.abcam.com/ps/products/247/ab247876/Images/ab247876-406149-anti-atg12-antibody-epr4800-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)All lanes : Anti-ATG12 antibody [EPR4800] (ab109491) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDaThis data was developed using ab109491, the same antibody clone in a different buffer formulation.
Observed band shows ATG12-ATG5 conjugation.
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![Western blot - Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)](https://www.abcam.com/ps/products/247/ab247876/Images/ab247876-402859-anti-atg12-antibody-epr4800-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)All lanes : Anti-ATG12 antibody [EPR4800] (ab109491) at 1/1000 dilution
Lane 1 : ATG12 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDaThis data was developed using ab109491, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)](https://www.abcam.com/ps/products/247/ab247876/Images/ab247876-1-anti-atg12-antibody-epr4800-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)This data was developed using ab109491, the same antibody clone in a different buffer formulation.ab109491, at 1/250 dilution, staining ATG12 in paraffin-embedded Human breast carcinoma by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)](https://www.abcam.com/ps/products/247/ab247876/Images/ab247876-5-benefits-of-recombinant-antibodies.png)
Anti-ATG12 antibody [EPR4800] - BSA and Azide free (ab247876)