Anti-ATF7 antibody (ab87844)
Key features and details
- Rabbit polyclonal to ATF7
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATF7 antibody
See all ATF7 primary antibodies -
Description
Rabbit polyclonal to ATF7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region within the C terminal amino acids 444-494 (ATAPSNGLSV RSAAEAVATS VLTQMASQRT ELSMPIQSHV IMTPQSQSAG R) of human ATF7 (CAA40483.1).
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified using an epitope specific to ATF7 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATF7 antibody (ab87844)All lanes : Anti-ATF7 antibody (ab87844) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunoprecipitation - Anti-ATF7 antibody (ab87844)Detection of human ATF7 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab87844 at 10 µg/mg lysate for IP. Subsequent Western blot detection used ab87844 at 1 µg/ml.
Developed using the ECL technique, with an exposure time of 10 seconds.
