Anti-ASF1A antibody (ab24171)
Key features and details
- Rabbit polyclonal to ASF1A
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ASF1A antibody -
Description
Rabbit polyclonal to ASF1A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human ASF1 alpha/beta.
Read Abcam's proprietary immunogen policy (Peptide available as ab37490.) -
Positive control
- Recombinant Human ASF1A protein (ab84211) can be used as a positive control in WB. ab24171 gives a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line) and MCF-7 (Human breast adenocarcinoma cell line).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications ChickenCowApplication Abreviews Notes ICC/IF Use a concentration of 1 µg/ml.WB 1/250. Detects a band of approximately 23, 33 kDa (predicted molecular weight: 23 kDa).Notes ICC/IF
Use a concentration of 1 µg/ml.WB
1/250. Detects a band of approximately 23, 33 kDa (predicted molecular weight: 23 kDa).Target
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Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the ASF1 family. -
Post-translational
modificationsPhosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 618099 Cow
- Entrez Gene: 25842 Human
- Omim: 609189 Human
- SwissProt: Q2KIG1 Cow
- SwissProt: Q9Y294 Human
- Unigene: 292316 Human
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Alternative names
- Anti silencing function 1A antibody
- Anti-silencing function protein 1 homolog A antibody
- ASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody
see all
Images
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Western blot - Anti-ASF1A antibody (ab24171)All lanes : Anti-ASF1A antibody (ab24171) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lane 4 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23,33 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific secondary antibody binding)
ASF1 typically shows up as a band at approximately 33 kDa band on Western blots but has a predicted molecular weight of 23kDa. Occasionally doublet or triplet bands can be observed due to phosphorylation differences. -
Immunocytochemistry/ Immunofluorescence - Anti-ASF1A antibody (ab24171)ICC/IF image of ab24171 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab24171, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
References (0)
ab24171 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-ASF1A antibody (ab24171)All lanes : Anti-ASF1A antibody (ab24171) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lane 4 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23,33 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific secondary antibody binding)
ASF1 typically shows up as a band at approximately 33 kDa band on Western blots but has a predicted molecular weight of 23kDa. Occasionally doublet or triplet bands can be observed due to phosphorylation differences. -
Immunocytochemistry/ Immunofluorescence - Anti-ASF1A antibody (ab24171)ICC/IF image of ab24171 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab24171, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).