Anti-ARTD10 antibody (ab70800)
Key features and details
- Rabbit polyclonal to ARTD10
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARTD10 antibody -
Description
Rabbit polyclonal to ARTD10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ARTD10 aa 300-350. NP_116178.1
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Positive control
- WB: Whole cell lysate from HeLa cells.
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General notes
Protein previously known as PARP10.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTD10 antibody (ab70800)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labeling ARTD10 with ab70800 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-ARTD10 antibody (ab70800)All lanes : Anti-ARTD10 antibody (ab70800) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 123 kDa
Observed band size: 123 kDa
Additional bands at: 60 kDa, 70 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Immunoprecipitation - Anti-ARTD10 antibody (ab70800)1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70800 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of immunoprecipitate was loaded per lane, and probed with ab70800 at 1ug/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.