Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Arp2 antibody (ab47654)

Key features and details

  • Rabbit polyclonal to Arp2
  • Suitable for: IP, WB, ICC/IF
  • Reacts with: Mouse, Human, Xenopus laevis
  • Isotype: IgG

Overview

  • Product name

    Anti-Arp2 antibody
    See all Arp2 primary antibodies

  • Description

    Rabbit polyclonal to Arp2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human, Xenopus laevis
    Predicted to work with: Rat, Chicken, Cow, Orangutan

  • Immunogen

    Synthetic peptide corresponding to Human Arp2 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab47653)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates; HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line), HEK293 (Human embryonic kidney cell line), HepG2 (Human hepatocellular liver carcinoma cell line), MCF7 (Human breast adenocarcinoma cell line), and SHSY-5Y (Human neuroblastoma cell line).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab47654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP (1)
Use at an assay dependent concentration.
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
Notes
IP
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

Target

Images

  • Western blot - Anti-Arp2 antibody (ab47654)
    Western blot - Anti-Arp2 antibody (ab47654)
    All lanes : Anti-Arp2 antibody (ab47654) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa
    Additional bands at: 27 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody (ab47654)
    Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody (ab47654)

    ICC/IF image of ab47654 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab47654, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells.

  • Immunoprecipitation - Anti-Arp2 antibody (ab47654)
    Immunoprecipitation - Anti-Arp2 antibody (ab47654)
    Arp2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to Arp2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab47654.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 45kDa: Arp2.

References (19)

Publishing research using ab47654? Please let us know so that we can cite the reference in this datasheet.

ab47654 has been referenced in 19 publications.

  • Mitrentsi I  et al. Heterochromatic repeat clustering imposes a physical barrier on homologous recombination to prevent chromosomal translocations. Mol Cell 82:2132-2147.e6 (2022). PubMed: 35447083
  • Aikemu B  et al. NDRG1 regulates Filopodia-induced Colorectal Cancer invasiveness via modulating CDC42 activity. Int J Biol Sci 17:1716-1730 (2021). PubMed: 33994856
  • Bessy T  et al. Hematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1. J Cell Biol 220:N/A (2021). PubMed: 34570198
  • Choi J  et al. Pimozide suppresses cancer cell migration and tumor metastasis through binding to ARPC2, a subunit of the Arp2/3 complex. Cancer Sci 110:3788-3801 (2019). PubMed: 31571309
  • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
View all Publications for this product

Images

  • Western blot - Anti-Arp2 antibody (ab47654)
    Western blot - Anti-Arp2 antibody (ab47654)
    All lanes : Anti-Arp2 antibody (ab47654) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa
    Additional bands at: 27 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody (ab47654)
    Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody (ab47654)

    ICC/IF image of ab47654 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab47654, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells.

  • Immunoprecipitation - Anti-Arp2 antibody (ab47654)
    Immunoprecipitation - Anti-Arp2 antibody (ab47654)
    Arp2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to Arp2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab47654.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 45kDa: Arp2.

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