Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ARH antibody [EPR13115] - N-terminal (ab181043)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13115] to ARH - N-terminal
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-ARH antibody [EPR13115] - N-terminal
See all ARH primary antibodies
Description

Rabbit monoclonal [EPR13115] to ARH - N-terminal
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Humab fetal liver, K562 and 293T cell lysates. K562 cells; mouse heart and kidney and rat heart and kidney tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13115
Isotype

IgG
Research areas

Western blot - Anti-ARH antibody [EPR13115] - N-terminal (ab181043)

All lanes : Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/1000 dilution

Lane 1 : Mouse heart tissue lysates
Lane 2 : Mouse kidney tissue lysates
Lane 3 : Rat heart tissue lysates
Lane 4 : Rat kidney tissue lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 34 kDa
Observed band size: 36 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Immunoprecipitation - Anti-ARH antibody [EPR13115] - N-terminal (ab181043)

Western blot analysis of ARH in immunoprecipitation pellets from K562. ab181043 used at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
Western blot - Anti-ARH antibody [EPR13115] - N-terminal (ab181043)

Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/1000 dilution + Human fetal liver lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1000 µg

Predicted band size: 34 kDa
Western blot - Anti-ARH antibody [EPR13115] - N-terminal (ab181043)

All lanes : Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/5000 dilution

Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysatae

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Anti-ARH antibody [EPR13115] - N-terminal (ab181043)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com