Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [3G3B9] to ARD1A
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Mouse, Human, African green monkey, Recombinant fragment
Isotype: IgG1

Product name

Anti-ARD1A antibody [3G3B9]
See all ARD1A primary antibodies
Description

Mouse monoclonal [3G3B9] to ARD1A
Host species

Mouse
Tested applications

Suitable for: WB, Flow Cyt, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Human, African green monkey, Recombinant fragment
Immunogen

Recombinant fragment corresponding to Human ARD1A aa 111-235. Expressed in E. coli.
Database link: P41227
Positive control
- Flow Cytometry: SMMC-7721 cells. ICC/IF: HeLa cells. WB: COS-7, HEK-293, HL-60, MCF7, HeLa, NIH/3T3, and C2C12 cell lysates; Recombinant human ARD1A (amino acids 111-235) protein; Human ARD1A (amino acids 111-235)-hIgGFc transfected HEK-293 cell lysate.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

3G3B9
Isotype

IgG1
Research areas

Western blot - Anti-ARD1A antibody [3G3B9] (ab234076)

All lanes : Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution

Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 6 : NIH-3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 7 : C2C12 (mouse myoblast cell line) cell lysate

Predicted band size: 26 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ARD1A antibody [3G3B9] (ab234076)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARD1A (green) using ab234076 at 1/200 dilution in ICC/IF.

Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AlexaFluor^®555-Phalloidin.
Flow Cytometry - Anti-ARD1A antibody [3G3B9] (ab234076)

Flow Cytometry analysis of SMMC-7721 (human hepatocarcinoma cell line) cells, labeling ARD1A with ab234076 at a 1/200 dilution (green) compared to a negative control (red).
Western blot - Anti-ARD1A antibody [3G3B9] (ab234076)

All lanes : Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Human ARD1A (amino acids 111-235)-hIgGFc transfected HEH-293 cell lysate

Predicted band size: 26 kDa
Western blot - Anti-ARD1A antibody [3G3B9] (ab234076)

Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution + Recombinant human ARD1A (amino acids 111-235) protein

Predicted band size: 26 kDa

Expected recombinant MW is 47.2 kDa

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