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Anti-ARD1A antibody [3G3B9] (ab234076)

Key features and details

  • Mouse monoclonal [3G3B9] to ARD1A
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human, African green monkey, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-ARD1A antibody [3G3B9]
    See all ARD1A primary antibodies

  • Description

    Mouse monoclonal [3G3B9] to ARD1A

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human, African green monkey, Recombinant fragment

  • Immunogen

    Recombinant fragment corresponding to Human ARD1A aa 111-235. Expressed in E. coli.
    Database link: P41227

  • Positive control

    • Flow Cytometry: SMMC-7721 cells. ICC/IF: HeLa cells. WB: COS-7, HEK-293, HL-60, MCF7, HeLa, NIH/3T3, and C2C12 cell lysates; Recombinant human ARD1A (amino acids 111-235) protein; Human ARD1A (amino acids 111-235)-hIgGFc transfected HEK-293 cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-ARD1A antibody [3G3B9] (ab234076)
    Western blot - Anti-ARD1A antibody [3G3B9] (ab234076)
    All lanes : Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution

    Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 3 : HL-60 (human promyelocytic leukemia cell line) cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 6 : NIH-3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 7 : C2C12 (mouse myoblast cell line) cell lysate

    Predicted band size: 26 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ARD1A antibody [3G3B9] (ab234076)
    Immunocytochemistry/ Immunofluorescence - Anti-ARD1A antibody [3G3B9] (ab234076)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARD1A (green) using ab234076 at 1/200 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AlexaFluor®555-Phalloidin. 

  • Flow Cytometry - Anti-ARD1A antibody [3G3B9] (ab234076)
    Flow Cytometry - Anti-ARD1A antibody [3G3B9] (ab234076)

    Flow Cytometry analysis of SMMC-7721 (human hepatocarcinoma cell line) cells, labeling ARD1A with ab234076 at a 1/200 dilution (green) compared to a negative control (red).

  • Western blot - Anti-ARD1A antibody [3G3B9] (ab234076)
    Western blot - Anti-ARD1A antibody [3G3B9] (ab234076)
    All lanes : Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : Human ARD1A (amino acids 111-235)-hIgGFc transfected HEH-293 cell lysate

    Predicted band size: 26 kDa

  • Western blot - Anti-ARD1A antibody [3G3B9] (ab234076)
    Western blot - Anti-ARD1A antibody [3G3B9] (ab234076)
    Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution + Recombinant human ARD1A (amino acids 111-235) protein

    Predicted band size: 26 kDa



    Expected recombinant MW is 47.2 kDa

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