Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19378] to Apolipoprotein E - BSA and Azide free
  • Suitable for: IHC-P, WB, IHC-Fr, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free
    See all Apolipoprotein E primary antibodies

  • Description

    Rabbit monoclonal [EPR19378] to Apolipoprotein E - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse plasma; Mouse liver, brain and kidney lysates; Rat plasma; Rat liver, brain and kidney lysates. IHC-P: Mouse cerebral cortex and liver tissues; Rat cerebral cortex and spleen tissues. IHC-Fr: Mouse cortex tissue. IP: Mouse plasma.

  • General notes

    Ab245999 is the carrier-free version of ab183596. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245999 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Western blot - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    Western blot - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    All lanes : Anti-Apolipoprotein E antibody [EPR19378] (ab183596) at 1/5000 dilution

    Lane 1 : Mouse plasma
    Lane 2 : Mouse liver lysate
    Lane 3 : Rat plasma
    Lane 4 : Rat liver lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Rat brain lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Mouse kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    This data was developed using ab183596, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-4: 4 seconds; Lanes 5-7: 15 seconds; Lane 8: 1 minute.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)

    This data was developed using ab183596, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on gliocytes of mouse cerebral cortex is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    This data was developed using ab183596, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on hepatocytes of mouse liver is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    This data was developed using ab183596, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on gliocytes of rat cerebral cortex is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    This data was developed using ab183596, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on macrophages of rat spleen is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Frozen sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    Immunohistochemistry (Frozen sections) - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    This data was developed using ab183596, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cortex tissue section labeling Apolipoprotein E with ab183596 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. The result showed cytoplasmic staining on mouse cortex. The nuclear counter stain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
  • Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    This data was developed using ab183596, the same antibody clone in a different buffer formulation.Apolipoprotein E was immunoprecipitated from 1mg of Mouse plasma with ab183596 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183596 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse plasma, 10µg (Input). Lane 2: ab183596 IP in Mouse plasma. Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab183596 in Mouse plasma. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
  • Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)
    Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Apolipoprotein E antibody [EPR19378] - BSA and Azide free (ab245999)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.