Anti-APOL2 antibody (ab241378)
Key features and details
- Rabbit polyclonal to APOL2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APOL2 antibody
See all APOL2 primary antibodies -
Description
Rabbit polyclonal to APOL2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human APOL2 aa 287-337. The exact sequence is proprietary.
Database link: Q9BQE5 -
Positive control
- WB: H226, HeLa, HEK-293T and jurkat whole cell lysate. IP: NCI-H226 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to APOL2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-APOL2 antibody (ab241378)All lanes : Anti-APOL2 antibody (ab241378) at 1/1000 dilution
Lane 1 : NCI-H226 whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 3 minutesLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-APOL2 antibody (ab241378)APOL2 was immunoprecipitated from NCI-H226 whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, prepared using NETN lysis buffer) using ab241378 at 8 µL per reaction. Western bot was performed on the immunoprecipitate using ab241378 at 1/1000 dilution.
Lane 1: ab241378 IP in NCI-H226 whole cell lysate.
Lane 2: Control IgG IP inNCI-H226 whole cell lysate.Detection: Chemiluminescence with an exposure time of 3 minutes.
Lysates prepared in NETN buffer.
