Anti-AP1S1 antibody (ab217055)
Key features and details
- Rabbit polyclonal to AP1S1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AP1S1 antibody -
Description
Rabbit polyclonal to AP1S1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman -
Immunogen
Synthetic peptide within Human AP1S1 aa 100 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P61966 -
Positive control
- HeLa, 293T, Jurkat, NIH 3T3, TCMK1 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab217055 was affinity purified using an epitope specific to AP1S1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-AP1S1 antibody (ab217055) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TCMK1 cell lysate
Lane 5 : NIH 3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa
Exposure time: 10 secondscells prepared using NETN lysis buffer
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Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling AP1S1 using ab217055 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated AP1S1, ab217055 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.