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Developmental Biology Lineage specification Ectoderm

Anti-Anterior Gradient 2 antibody [IMG10E2] (ab189361)

Price and availability

335 040 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Anterior Gradient 2 antibody [IMG10E2] (ab189361)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [IMG10E2] to Anterior Gradient 2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Anterior Gradient 2 antibody [IMG10E2]
    See all Anterior Gradient 2 primary antibodies
  • Description

    Mouse monoclonal [IMG10E2] to Anterior Gradient 2
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Anterior Gradient 2 aa 50-150 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_006399.1
    Database link: O95994

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human small intestine tissue; Human stomach, mouse stomach and rat stomach tissue lysate; HCT-116 cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    IMG10E2
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Cancer
    • Tumor biomarkers
    • Other
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Images

  • Western blot - Anti-Anterior Gradient 2 antibody [IMG10E2] (ab189361)
    Western blot - Anti-Anterior Gradient 2 antibody [IMG10E2] (ab189361)
    All lanes : Anti-Anterior Gradient 2 antibody [IMG10E2] (ab189361) at 5 µg/ml

    Lane 1 : Human stomach lysate in the absence of immunizing peptide
    Lane 2 : Human stomach tissue lysate in the presence of immunizing peptide
    Lane 3 : mouse stomach tissue lysate
    Lane 4 : rat stomach tissue lysate
    Lane 5 : HCT-116 cell lysate

    Secondary
    All lanes : Goat anti-Mouse Ig HRP

    Developed using the ECL technique.

    Predicted band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [IMG10E2] (ab189361)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [IMG10E2] (ab189361)

    Immunohistochemical analysis of formalin fixed, paraffin embedded Human small intestine tissue labeling Anterior Gradient 2 with ab189361 at 10 µg/ml.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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