Anti-ANKRD10 antibody (ab204396)
Key features and details
- Rabbit polyclonal to ANKRD10
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ANKRD10 antibody -
Description
Rabbit polyclonal to ANKRD10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ANKRD10 aa 100-250.
Database link: Q9NXR5 -
Positive control
- WB: RT4 and U-251 MG whole cell lysate; IHC-P: Human stomach tissue; ICC: U2 OS whole cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ANKRD10 antibody (ab204396)
Immunocytochemical analysis of permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling ANKRD10 in the cytoplasm and microtubules (green) with ab204396 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD10 antibody (ab204396)Immunohistochemical analysis of human stomach tissue labeling ANKRD10 in the shows cytoplasm of glandular cells with ab204396 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-ANKRD10 antibody (ab204396)All lanes : Anti-ANKRD10 antibody (ab204396) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Predicted band size: 45 kDa