Anti-ANKLE2 antibody (ab225905)
Key features and details
- Rabbit polyclonal to ANKLE2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ANKLE2 antibody
See all ANKLE2 primary antibodies -
Description
Rabbit polyclonal to ANKLE2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human ANKLE2 aa 800-850. The exact sequence is proprietary. (NP_055929.1).
Database link: Q86XL3 -
Positive control
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab225905 was affinity purified using an epitope specific to ANKLE2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ANKLE2 antibody (ab225905)All lanes : Anti-ANKLE2 antibody (ab225905) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 104 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-ANKLE2 antibody (ab225905)ANKLE2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225905 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225905 at 1 µg/ml.
Lane 1: ab225905 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
