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Cell Biology Other Antibodies

Anti-RNF214 antibody [EPR12940] (ab180850)

Price and availability

264 681 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-RNF214 antibody [EPR12940] (ab180850)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12940] to RNF214
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-RNF214 antibody [EPR12940]
    See all RNF214 primary antibodies
  • Description

    Rabbit monoclonal [EPR12940] to RNF214
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human RNF214. The exact sequence is proprietary.
    Database link: Q8ND24

  • Positive control

    • Human fetal brain, HT-1376 and HepG2 lysates; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12940
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-RNF214 antibody [EPR12940] (ab180850)
    Immunocytochemistry/ Immunofluorescence - Anti-RNF214 antibody [EPR12940] (ab180850)

    Immunofluorescence analysis of HepG2 cells labeling RNF214 using ab180850 at 1/50 dilution.

  • Western blot - Anti-RNF214 antibody [EPR12940] (ab180850)
    Western blot - Anti-RNF214 antibody [EPR12940] (ab180850)
    All lanes : Anti-RNF214 antibody [EPR12940] (ab180850) at 1/1000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : HT-1376 lysates
    Lane 3 : HepG2 lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 78 kDa

  • Immunoprecipitation - Anti-RNF214 antibody [EPR12940] (ab180850)
    Immunoprecipitation - Anti-RNF214 antibody [EPR12940] (ab180850)

    Western blot analysis on immunoprecipitation pellet from HT-1376 cell lysate labeling RBF214 using ab180850 at 1/10 dilution.

  • Anti-RNF214 antibody [EPR12940] (ab180850)
    Anti-RNF214 antibody [EPR12940] (ab180850)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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