Anti-Angiopoietin 1 + Angiopoietin 2 antibody (ab65835)
Key features and details
- Rabbit polyclonal to Angiopoietin 1 + Angiopoietin 2
- Suitable for: ICC/IF, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Angiopoietin 1 + Angiopoietin 2 antibody -
Description
Rabbit polyclonal to Angiopoietin 1 + Angiopoietin 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatWB Rat
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Immunogen
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Positive control
- WB: Rat brain tissue lysate. ICC: PC12 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Angiopoietin 1 + Angiopoietin 2 antibody (ab65835)Anti-Angiopoietin 1 + Angiopoietin 2 antibody (ab65835) at 2 µg/ml + rat brain tissue lysate
Predicted band size: 57 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin 1 + Angiopoietin 2 antibody (ab65835)ICC/IF image of ab65835 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65835, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Angiopoietin 1 + Angiopoietin 2 antibody (ab65835)