Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (ab45089)
![Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (ab45089)](https://www.abcam.com/ps/products/45/ab45089/Images/ab45089-57219-anti-androgen-receptor-phospho-s210-s213-antibody-156c1352-western-blot.jpg "Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (ab45089)")
Key features and details
- Mouse monoclonal [156C135.2] to Androgen Receptor (phospho S210 + S213)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2]
See all Androgen Receptor primary antibodies -
Description
Mouse monoclonal [156C135.2] to Androgen Receptor (phospho S210 + S213) -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Hamster, Cat, Dog, Pig, Chimpanzee, Monkey, Baboon, Cynomolgus monkey, Hedgehog, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Human Androgen Receptor aa 200-300 (phospho S213).
Database link: A39248 -
Positive control
- IGF1 stimulated LNCaP cells. IHC-P: human normal prostate FFPE tissue sections
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General notes
In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. The serine phosphorylation site recognized by ab45089 has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. The serine phosphorylation site recognized by ab45089 has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen. -
Clonality
Monoclonal -
Clone number
156C135.2 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (ab45089)All lanes : Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (ab45089) at 1 µg/ml
Lane 1 : LNCaP cells with serum starved cells
Lane 2 : LNCaP cells with IGF1 incubated for 4h at 0.1 µg/ml
Lane 3 : LNCaP cells with incubated with 20 ug LY294002 for 30 min then IGF1 incubated for 4h at 0.1 µg/ml
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (ab45089)](https://www.abcam.com/ps/products/45/ab45089/Images/ab45089-57218-anti-androgen-receptor-phospho-s210-s213-antibody-156c1352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (ab45089)IHC image of Androgen Receptor (phospho S213 + S210) staining in human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45089, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
