Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5119(2)] to Amyloid Precursor Protein - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free
    See all Amyloid Precursor Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR5119(2)] to Amyloid Precursor Protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248567 is the carrier-free version of ab133588 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248567 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)

    This data was developed using ab133588, the same antibody clone in a different buffer formulation.

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)

    Lane 2: Amyloid Precursor Protein knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HepG2 whole cell lysate (20 µg)

    Lane 4: HeLa whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133588 observed at 110 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab133588 was shown to recognize Amyloid Precursor Protein when Amyloid Precursor Protein knockout samples were used, along with additional cross-reactive bands. Wild-type and Amyloid Precursor Protein knockout samples were subjected to SDS-PAGE. ab133588 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    Immunoprecipitation - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)

    This data was developed using ab133588, the same antibody clone in a different buffer formulation.

    ab133588 (purified) at 1/20 immunoprecipitating amyloid precursor protein in human fetal brain (Lane 2). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
  • Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/20000 dilution (purified) + Human fetal brain tissue lysate

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 86 kDa



    This data was developed using ab133588, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/2000 dilution (purified) + HEK-293 cell lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 86 kDa



    This data was developed using ab133588, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/2000 dilution (purified) + SH-SY5Y cell lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 86 kDa



    This data was developed using ab133588, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    All lanes : Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : U87 MG cell lysate
    Lane 4 : Human fetal brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 86 kDa
    Observed band size: 100-120 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133588, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    OI-RD Scanning - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    This data was developed using ab133588, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)
    Anti-Amyloid Precursor Protein antibody [EPR5119(2)] - BSA and Azide free (ab248567)

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