Antibodies, Proteins, Kits and Reagents for Life Science

Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)

Key features and details

Mouse monoclonal [AMACR/1864] to AMACR - BSA and Azide free
Suitable for: IHC-P, Protein Array, WB
Reacts with: Human, Recombinant fragment
Isotype: IgG1

Product name

Anti-AMACR antibody [AMACR/1864] - BSA and Azide free
See all AMACR primary antibodies
Description

Mouse monoclonal [AMACR/1864] to AMACR - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: IHC-P, Protein Array, WBmore details
Species reactivity

Reacts with: Human, Recombinant fragment
Immunogen

Recombinant fragment within Human AMACR. The exact sequence is proprietary.
Database link: Q9UHK6
Positive control
- WB: Human kidney lysate; PC-3 cell lysate; Recombinant AMACR protein. IHC-P: Human prostate carcinoma and renal cell carcinoma tissues.
General notes

ab268183 is the PBS only version of ab268062.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from bioreactor concentrate.
Clonality

Monoclonal
Clone number

AMACR/1864
Isotype

IgG1
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for AMACR using ab268062 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)
Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)

Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/ml + Human kidney tissue lysate

Predicted band size: 42 kDa

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for AMACR using ab268062 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)
Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)

Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/ml + PC-3 (human prostate adenocarcinoma cell line) cell lysate

Predicted band size: 42 kDa

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)
Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)

All lanes : Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/ml

Lane 1 : Recombinant AMACR protein
Lane 2 : Human kidney lysate

Predicted band size: 42 kDa

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)
Protein Array - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)

Protein Array containing more than 19,000 full-length human proteins using ab268062.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)

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