Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)
![Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)](https://www.abcam.com/ps/products/280/ab280377/Images/ab280377-456803-anti-alphasynuclein-antibody-42asynuclein-western-blot-mouse-cerebellum-lysate10-ug.jpg "Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [42/a-Synuclein] to Alpha-synuclein
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Alpha-synuclein antibody [42/a-Synuclein]
See all Alpha-synuclein primary antibodies -
Description
Mouse monoclonal [42/a-Synuclein] to Alpha-synuclein -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- WB: Mouse cerebellum, Human cerebellum, Rat hippocampus. IHC-P: Rat cerebrum, Mouse cerebrum, Human cerebrum.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
42/a-Synuclein -
Isotype
IgG1
Images
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Western blot - Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)All lanes : Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377) at 1/1000 dilution
Lane 1 : Mouse cerebellum lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Human cerebellum lysate
Lane 4 : Human kidney lysate
Lane 5 : Rat hippocampus lysate
Lane 6 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Negative control: mouse kidney, human kidney, rat kidney (PMID:14997013)
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:14997013, 30553024).
Exposure time 15 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)](https://www.abcam.com/ps/products/280/ab280377/Images/ab280377-3-anti-alphasynuclein-antibody-42asynuclein-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Alpha-synuclein with abab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in human cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)](https://www.abcam.com/ps/products/280/ab280377/Images/ab280377-2-anti-alphasynuclein-antibody-42asynuclein-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Alpha-synuclein with abab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in mouse cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)](https://www.abcam.com/ps/products/280/ab280377/Images/ab280377-1-anti-alphasynuclein-antibody-42asynuclein-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Alpha-synuclein with abab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in rat cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)](https://www.abcam.com/ps/products/280/ab280377/Images/ab280377-4-Benefits-of-Recombinant-antibodies.jpg)
Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377)