Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
![Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)](https://www.abcam.com/ps/products/119/ab119952/Images/ab119952-133549-anti-alpha-smooth-muscle-actin-antibody-4a4-immunohistochemistry.jpg "Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)")
Key features and details
- Mouse monoclonal [4A4] to alpha smooth muscle Actin
- Suitable for: WB, ELISA, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-alpha smooth muscle Actin antibody [4A4]
See all alpha smooth muscle Actin primary antibodies -
Description
Mouse monoclonal [4A4] to alpha smooth muscle Actin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide of Human alpha smooth muscle Actin (C-EYDEAGPSIVHRKCF) conjugated KLH
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Positive control
- Recombinant alpha smooth muscle Actin protein, Hela, A431, Jurkat, K562, HEK293, HepG2, NIH3T3, PC12 and COS7 cell lysates, HepG2 cells, HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4A4 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)Immunohistochemical analysis of paraffin-embedded Human duodenum tissue using ab119952 at a dilution of 1/200 with DAB staining.
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![Western blot - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)](https://www.abcam.com/ps/products/119/ab119952/Images/ab119952-133550-anti-alpha-smooth-muscle-actin-antibody-4a4-western-blot.jpg)
Western blot - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)All lanes : Anti-alpha smooth muscle Actin antibody [4A4] (ab119952) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HepG2 cell lysate
Lane 7 : NIH3T3 cell lysate
Lane 8 : PC12 cell lysate
Lane 9 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Predicted band size: 42 kDa
Observed band size: 42 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)](https://www.abcam.com/ps/products/119/ab119952/Images/ab119952-133548-anti-alpha-smooth-muscle-actin-antibody-4a4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)Immunohistochemical analysis of paraffin-embedded Human esophagus tissue using ab119952 at a dilution of 1/200 with DAB staining. -
![Flow Cytometry - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)](https://www.abcam.com/ps/products/119/ab119952/Images/ab119952-133546-anti-alpha-smooth-muscle-actin-antibody-4a4-flow-cytometry.jpg)
Flow Cytometry - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)Flow cytometric analysis of Hela cells using ab119952 at a dilution of 1/200 (green) and negative control (purple). -
![ELISA - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)](https://www.abcam.com/ps/products/119/ab119952/Images/ab119952-133545-anti-alpha-smooth-muscle-actin-antibody-4a4-elisa.jpg)
ELISA - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)ELISA, using ab119952 at a dilution of 1/10000.
