Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALDH1A2 antibody (ab75674)

Key features and details

  • Rabbit polyclonal to ALDH1A2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-ALDH1A2 antibody
    See all ALDH1A2 primary antibodies

  • Description

    Rabbit polyclonal to ALDH1A2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Monkey

  • Immunogen

    Synthetic peptide corresponding to Human ALDH1A2 aa 450 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101078)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab75674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (7)
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently.

  • Pathway

    Cofactor metabolism; retinol metabolism.

  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AL1A2_HUMAN antibody
    • Aldehyde dehydrogenase family 1 member A2 antibody
    • ALDH1A2 aldehyde dehydrogenase 1 family, member A2 antibody
    • ALDH1A2 antibody
    • Aldh1a7 antibody
    • AV116159 antibody
    • MGC26444 antibody
    • RALDH 2 antibody
    • RALDH(II) antibody
    • Raldh1 antibody
    • RalDH2 antibody
    • RALDH2 T antibody
    • Retinal dehydrogenase 2 antibody
    • Retinaldehyde dehydrogenase 2 antibody
    • Retinaldehyde specific dehydrogenase type 2 antibody
    • Retinaldehyde-specific dehydrogenase type 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (ab75674)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (ab75674)
    IHC image of ab75674 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75674, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-ALDH1A2 antibody (ab75674)
    Western blot - Anti-ALDH1A2 antibody (ab75674)
    All lanes : Anti-ALDH1A2 antibody (ab75674) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 17 kDa, 46 kDa, 73 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes

References (12)

Publishing research using ab75674? Please let us know so that we can cite the reference in this datasheet.

ab75674 has been referenced in 12 publications.

  • Meuser M  et al. FGFR2 signaling enhances the SHH-BMP4 signaling axis in early ureter development. Development 149:N/A (2022). PubMed: 35020897
  • Li Y  et al. Inhibition of GSK3 Represses the Expression of Retinoic Acid Synthetic Enzyme ALDH1A2 via Wnt/ß-Catenin Signaling in WiT49 Cells. Front Cell Dev Biol 8:94 (2020). PubMed: 32258025
  • Sun J  et al. Histone demethylase LSD1 is critical for endochondral ossification during bone fracture healing. Sci Adv 6:N/A (2020). PubMed: 33148658
  • Kim EW  et al. Vitamin A Metabolism by Dendritic Cells Triggers an Antimicrobial Response against Mycobacterium tuberculosis. mSphere 4:N/A (2019). PubMed: 31167948
  • Landrock KK  et al. Pleiotropic neuropathological and biochemical alterations associated with Myo5a mutation in a rat Model. Brain Res 1679:155-170 (2018). PubMed: 29217155
View all Publications for this product

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (ab75674)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (ab75674)
    IHC image of ab75674 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75674, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-ALDH1A2 antibody (ab75674)
    Western blot - Anti-ALDH1A2 antibody (ab75674)
    All lanes : Anti-ALDH1A2 antibody (ab75674) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 17 kDa, 46 kDa, 73 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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