Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15425(B)] to Aldehyde dehydrogenase 10 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free
    See all Aldehyde dehydrogenase 10 primary antibodies

  • Description

    Rabbit monoclonal [EPR15425(B)] to Aldehyde dehydrogenase 10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, A549, 293T and U87-MG cell lysates; A549 and HepG2 cells.

  • General notes

    Ab250728 is the carrier-free version of ab184171. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250728 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
    Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
    All lanes : Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ALDH3A2 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab184171).

    Lanes 1 - 2: Merged signal (red and green). Green - ab184171 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab184171 was shown to react with ALDH3A2 in wild-type HeLa cells in Western blot with loss of signal observed in ALDH3A2 knockout cell line ab265427 (ALDH3A2 knockout cell lysate ab257829). Wild-type and ALDH3A2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab184171 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
    Immunocytochemistry - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
    This data was developed using ab184171, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of 4% paraformaldehyde fixed A549 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/250 dilution (red). A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue).
  • Flow Cytometry - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
    Flow Cytometry - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
    This data was developed using ab184171, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde fixed HepG2 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/170 dilution (red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Isotype control: Rabbit monoclonal IgG (green).
  • Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
    Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
    Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/10000 dilution + U87-MG cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa



    This data was developed using ab184171, the same antibody clone in a different buffer formulation.

     

  • Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
    Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
    All lanes : Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/2000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa



    This data was developed using ab184171, the same antibody clone in a different buffer formulation.

  • Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
    Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)

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