Anti-AlaRS antibody - C-terminal (ab226217)
Images
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Western blot - Anti-AlaRS antibody - C-terminal (ab226217)All lanes : Anti-AlaRS antibody - C-terminal (ab226217) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 107 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-AlaRS antibody - C-terminal (ab226217)AlaRS was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226217 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226217 at 0.4 µg/ml.
Lane 1: ab226217 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds
