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Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-AL-1 antibody (ab70114)

Price and availability

301 536 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-AL-1 antibody (ab70114)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to AL-1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-AL-1 antibody
    See all AL-1 primary antibodies
  • Description

    Rabbit polyclonal to AL-1
  • Host species

    Rabbit
  • Specificity

    ab70114 detects endogenous levels of total AL-1 protein.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human AL-1 (internal sequence).

  • General notes

    Previously labelled as Ephrin A5.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab70114 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Neuroscience
    • Sensory System
    • Visual system
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AL-1 antibody (ab70114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AL-1 antibody (ab70114) This image is courtesy of an anonymous abreview.

    ab70114 staining anti-AL-1 in wild-type and knockout mouse inferior colliculus/cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/200 in 1 % TBS/BSA buffer) for 2 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-AL-1 antibody (ab70114)
    Immunocytochemistry/ Immunofluorescence - Anti-AL-1 antibody (ab70114)

    ab70114 at 1/500 dilution staining AL-1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AL-1 antibody (ab70114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AL-1 antibody (ab70114)

    ab70114 at 1/50 dilution staining AL-1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide

  • Western blot - Anti-AL-1 antibody (ab70114)
    Western blot - Anti-AL-1 antibody (ab70114)
    All lanes : Anti-AL-1 antibody (ab70114) at 1/500 dilution

    Lane 1 : HeLa cell extracts
    Lane 2 : HeLa cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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